Stefan G Stanciu, Radu Hristu, George A Stanciu, Denis E Tranca, Lucian Eftimie, Adrian Dumitru, Mariana Costache, Harald A Stenmark, Harm Manders, Amit Cherian, Mariliis Tark-Dame, Erik M M Manders | Proc Natl Acad Sci U S A 2022 Nov 22;119(47):e2214662119
Summary
Second harmonic generation microscopy (SHG) is a powerful label-free technique for three-dimensional imaging of biological tissues and advanced materials, widely used for collagen visualization. However, progress in super-resolved SHG imaging has lagged behind fluorescence-based nanoscopy methods.
In this study, the authors demonstrated super-resolved re-scan SHG microscopy and showed that it provides resolution improvements beyond the conventional diffraction limit in collagen-rich tissues.
They also introduced super-resolved re-scan two-photon excited fluorescence microscopy, extending re-scan imaging principles to another nonlinear optical modality.
These findings highlight new opportunities for high-resolution label-free and multiphoton imaging in biological and materials research.
Read the publication: https://pubmed.ncbi.nlm.nih.gov/36375085