Jasna Simonović Radosavljević, Aleksandra Lj. Mitrović, Ksenija Radotić, László Zimányi,Győző Garab and Gábor Steinbach | Int J Mol Sci 2021 Jul 17;22(14):7661. doi: 10.3390/ijms22147661
Summary
Modern light microscopy techniques have greatly improved the understanding of plant cell ultrastructure and organelles. Laser-scanning and digital light microscopy offer high sensitivity, fast image acquisition, versatile 2D-4D analysis, and the ability to combine imaging with spectroscopic measurements.
In this review, the authors focus on differential polarization (DP) imaging and its applications in plant cell walls and chloroplasts. They describe how laser-scanning microscopes and the enhanced-resolution Re-scan Confocal Microscope (RCM) from Confocal NL can be fitted with DP attachments to measure polarization parameters alongside conventional imaging.
The review also presents examples of anisotropic molecular organization in chloroplast thylakoid membranes and wood cell walls, supported by quantitative analysis. Finally, the authors discuss future developments of micro-spectropolarimetry imaging for plant cell research.
Read more: https://pubmed.ncbi.nlm.nih.gov/34299279