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RCM

Sharp and sensitive

RCM Confocal NL | Discover super resolution imaging with Re-scan Confocal Microscope

Gentle live cell imaging

RCM is our second-generation RCM with digital scanner technology. It makes re-scanning the standard and allows a speed of 2fps at 512×512 pixels. RCM has optics to make it suitable for super-resolution imaging with high NA objectives in the low magnification range, like 40x 1.4. A lower magnification allows for a bigger field of view (FOV), brighter images, and even lower laser power. RCM has demonstrated imaging at 10 nano-watt excitation power!

The improvements of sCMOS cameras allow you to sample resolution of low magnification objectives effectively, without increasing the exposure time. In a regular PMT-based confocal this is not possible.

Discover super resolution imaging with RCM

Capture datasets of 170nm resolution raw (120 after deconvolution) over a very large FOV. Study fast live-cell dynamics and perform 3D imaging in optimized conditions. The increased detection efficiency facilitates acquisition in low fluorescence conditions, like single-molecule detection (smFISH).

In combination with silicon objectives, RCM allows for deep 3D imaging of organoids, zebrafish embryos, or larger live samples.

The benefits of RCM

Using RCM with a 40x 1.4 objective, you can see more cells at full resolution at once. A larger field of view increases the chances of getting the results you need.

Obtain sharp images with a high signal-to-noise ratio even in samples with a low amount of epitopes or weak stainings. Get more from your samples.

Use even lower laser power to minimize phototoxicity and photobleaching during live-cell imaging.

Getting super-resolution raw images, without averaging or integration, reduces the acquisition time and allows for a more precise analysis of the subcellular structures.

Neurons in co-culture stained for Actin (red), MAP2 (magenta) and Tau (green). Imaged on RCM2 with a 40x 1.4 objective. Sample courtesy: Vera Wiersma, VU University, Amsterdam, the Netherlands.

120nm

Super-resolution with 40x 1.4NA objectives

Large FOV

without increasing acquisition time

Add-on

Excellent add-on to any widefield microscope

Ease of use

Hardware integration in third-party software

Systems

RCM technical specs

RCM

$ 99

Per Month
Detector

Camera (sCMOS)


Resolution in real time

120 nm deconvolved, 170 nm raw image

Detector sensitivity
Up to 96% QE

FOV
FN12: 220×220 µm (40x objective)
FN18 when not using super resolution

Speed in line scanning
2 fps at 512 x 512 px, 15 fps in sprint level max at 256 x 256 px

Wavelength
Standard VIS (400-700 nm)

Software
Micromanager, Inscoper, Volocity, NIS Elements, SDK available for integration on request

Deconvolution
Microvolution (real time); SVI Hyugens (post processing)

Modalities
Super Resolution, Confocal, Widefield, Brightfield

Emission filter
Standard quad band, optional external single band filter wheel

Weight
23kg

RCM2.5

$ 99

Per Month
Detector

Camera (sCMOS)


Resolution in real time

120 nm deconvolved, 170 nm raw image

Detector sensitivity
Up to 96% QE

FOV
FN12: 220×220 µm (40x objective)
FN18 when not using super resolution

Speed in line scanning
2 fps at 512 x 512 px, 15 fps in sprint level max at 256 x 256 px

Wavelength
Standard VIS (400-700 nm), optional VIS-NIR (400-950 nm)

Software
Micromanager, Inscoper, Volocity, NIS Elements, SDK available for integration on request

Deconvolution
Microvolution (real time); SVI Hyugens (post processing)

Modalities
Super Resolution, Confocal, Widefield, Brightfield

Emission filter
Standard quad band, optional external single band filter wheel

Weight
23kg

Testimonials

Satisfied researchers
around the globe

Prof. Erwin PetermanFaculty of Science, Physics of Living Systems, Vrije Universiteit Amsterdam, The Netherlands

The NL5 has enabled us to image deep in different parts of the C.elegans that we could not so far measure properly.

Gabor CsucsETH Zürich

RCM is a very easy to use system that we have in combination with STORM and it provides great underlay images for STORM data.

Prof. Erwin PetermanFaculty of Science, Physics of Living Systems, Vrije Universiteit Amsterdam, The Netherlands

The NL5 has enabled us to image deep in different parts of the C.elegans that we could not so far measure properly.

Gabor CsucsETH Zürich

RCM is a very easy to use system that we have in combination with STORM and it provides great underlay images for STORM data.

Resources

Related Resources

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