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AION

The new standard in confocal microscopy

The most gentle 3D live cell imaging

AION is the third generation of our fast Line REscan confocal technology. It provides high-contrast images from thicker specimens such as organoids, tissue samples, plant and animal model organisms. The gentle conditions for your live samples allow AION to excel in long-time-lapse experiments and imaging low-signal samples.

The combination of camera-based detection and a set of filters allows you to acquire your images in both visible (VIS) and near-infrared (NIR). This gives you an improved temporal resolution, high sensitivity and unprecedented signal-to-noise ratio. You get even faster imaging with cleaner results, thanks to additional motorized single-band emission filters.

Double the FOV - Double the observation

AION is a confocal system that allows a 25 mm field of view (FOV) with a motorized pinhole that ensures spot-on confocality every time. 

Our system uses a line illumination through a slit pinhole, instead of a point illumination found in point scanner confocal microscopes. This provides high temporal resolution while reducing phototoxicity. Since phototoxicity is one of the biggest challenges for researchers, this makes AION the perfect tool for live cell imaging.

You can also add AION to any widefield microscope. This will turn it into an advanced and fast scanning confocal imaging system.

Fixed human fibroblast cells imaged with 100x objective. Nucleus – DAPI (blue), mitochondria – Tom20-Alexa488 (green), microtubules – Tubulin-Alexa555 (red). Scale bar 20 μm.

FN26.5

Double FOV size

140fps

Double acquisition speed

16x

More information acquired

VIS/NIR

Perfected for both VIS & NIR

Accelerate your research with the AION

A combination of low laser power requirements, high sensitivity of the detector and a novel optical design present in AION Line REscan enables imaging beyond 500μm of depth. This makes AION Line REscan confocal a perfect solution for live cell confocal microscopy of even thicker specimens like cleared tissue, whole zebrafish embryos, organoids and spheroids.

Optic tectum of Zebrafish. Image courtesy of Bram Willems (Brinks Lab, TU Delft).

Explore the benefits

Our flagship AION has a switchable pinhole, ensuring flexibility, sensitivity and optimal confocality.

Nyquisting every objective and adding fast multicolour imaging over a large FOV gives raise to the most light-efficient confocal system on the market.

Systems

Discover AION systems that meet your needs

AION α

$ 99

Per Month
Detector

Camera (sCMOS)


Resolution in real time

170 nm with deconvolution; 240 nm raw

Detector sensitivity
Up to 95% QE

FOV
26.5 mm (FN26.5), 450×450 µm (40x objective)

Speed
>140 fps @ 3Kx3K 

Speed in fixed line mode
>1000 fps (camera limited)

Wavelength
400-1100 nm (VIS + NIR)

Software
KRONOS, ZEN, Micromanager, SDK available for integration on request

Deconvolution
SVI Hyugens (post processing)

Modalities
Brightfield, Widefield and Confocal

Widefield/confocal switch
Motorized

Camera ports
1 or 2

Laser fiber inputs
2

Pinhole
Pre-selected

Dichroic
Pre-selected

AION λ

$ 99

Per Month
Detector

Camera (sCMOS)


Resolution in real time

170 nm with deconvolution; 240 nm raw

Detector sensitivity
Up to 95% QE

FOV
26.5 mm (FN26.5), 450×450 µm (40x objective)

Speed
>140 fps @ 3Kx3K 

Speed in fixed line mode
>1000 fps (camera limited)

Wavelength
400-1100 nm (VIS + NIR)

Software
KRONOS, ZEN, Micromanager, SDK available for integration on request

Deconvolution
SVI Hyugens (post processing)

Modalities
Brightfield, Widefield and Confocal

Widefield/confocal switch
Motorized

Camera ports
1 or 2

Laser fiber inputs
2

Pinhole
Motorized

Dichroic
Pre-selected

AION Σ

$ 99

Per Month
Detector

Camera (sCMOS)


Resolution in real time

170 nm with deconvolution; 240 nm raw

Detector sensitivity
Up to 95% QE

FOV
26.5 mm (FN26.5), 450×450 µm (40x objective)

Speed
>140 fps @ 3Kx3K 

Speed in fixed line mode
>1000 fps (camera limited)

Wavelength
400-1100 nm (VIS + NIR)

Software
KRONOS, ZEN, Micromanager, SDK available for integration on request

Deconvolution
SVI Hyugens (post processing)

Modalities
Brightfield, Widefield and Confocal

Widefield/confocal switch
Motorized

Camera ports
1 or 2

Laser fiber inputs
2

Pinhole
Motorized

Dichroic
Motorized

Resources

Related Resources

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