Manders, E. (2018) | Optik & Photonik, 13(3), 30–32
Summary
Confocal microscopy is widely recognized for its optical sectioning capability, delivering strong axial resolution and clear imaging from specific focal planes. A lesser-known advantage is its potential for improved lateral resolution beyond the classical diffraction limit described by Abbe’s law.
Traditionally, achieving this higher lateral resolution required sacrificing fluorescence signal, often resulting in noisier images with reduced sensitivity.
In this study, the authors showed that this limitation can be overcome through a redesigned optical approach known as re-scan confocal microscopy (RCM).
By using signal more efficiently while improving image resolution, RCM enables lateral super-resolution without the same loss of image quality seen in conventional methods.
Read the publication: https://doi.org/10.1002/opph.201800027